A fully funded PhD position. The proposed PhD project will study the pathways activated by the DNA sensors IFI16 and cGAS. It will focus on understanding the mechanism of sensing MPyV and human BKPyV genomes by DNA sensor p204/IFI16 and uncovering the role of cGAS association with polyomavirus minichromosomes in the cell nucleus. Confocal, super resolution and immunoelectron microscopy will be used to follow i) dynamics and spatial and functional organisation of IFI16/p204 and cGAS interactions, ii) IFI16/p204 and STING dynamics of trafficking after their activation and iii) the possible interaction of cGAS with viral nucleosomes as well as with cellular SMC5/6 complex and other proteins associated with viral genomes during replication and DNA damage response. Western blot, co-immunoprecipitation, pulldown assays and mass spectroscopy will be used for further identification of cGAS and p204/ IFI16 interacting partners and for studies of posttranslational modifications of IFI16, cGAS and STING. The extension of research to human BKPyV will be facilitated by exploiting newly available cell lines recently proposed as reservoir of BKPyV instead of primary human kidney cells.
Tagged as: Life Sciences