Doctoral researcher – Molecular and Developmental Biology, project title: Deciphering endocrine pancreas development and function at the single-cell level Full-time

Laboratory of Molecular Pathogenetics at the Institute of Biotechnology of the Czech Academy of Sciences announces an open competition in accordance with the Act No. 283/1992 Coll. on the Czech Academy of Sciences, and the Statutes of the Czech Academy of Sciences for a position:

Doctoral researcher – Molecular and Developmental Biology

Project title: Deciphering endocrine pancreas development and function at the single-cell level

Understanding the different ways in which epigenetic signals and transcription factor networks govern self-renewal, cell specification, differentiation, and reprogramming is a key step for advances in cell-based therapy and perspectives for medicine. Using mouse models, we aim to investigate how elimination and miss-expression of selected transcription factors affect epigenetic landscapes and downstream targets during embryonic development, particularly pancreatic development. We will use Cre/loxP and global gene expression profiling (RNA sequencing), single cell sequencing, CUT&RUN chromatin profiling, FACS, confocal microscopy, immunohistochemistry, and in situ hybridization to evaluate molecular phenotypes and regulatory mechanisms.

Requirements:

• The candidate should hold a Master degree or an equivalent degree in cell biology, genetics, biomedicine, molecular biology or related sciences (as of May 2025).
• The candidate must be willing to work with murine models.
• Good command of written and spoken English
• Prior experience working with mice, microdissections, fluorescence microscopic methods, and knowledge of molecular methods and developmental biology and/or bioinformatics is a plus.
• Teamwork, enthusiasm, and excellent communication skills

We offer to work in a creative, multidisciplinary international team. The laboratory is a part of the Institute of Biotechnology (IBT, www.ibt.cas.cz) located at BIOCEV, providing an inspiring multi-disciplinary research environment and access to state-of-the-art technologies and instruments. IBT/BIOCEV brings together > 500 scientists and students in > 50 research groups. Our laboratory collaborates with a number of European and US laboratories. The salary is in accordance with the collective labour agreement of the Institute of Biotechnology CAS. We provide excellent training conditions for PhD students.

S(he) need to successfully complete the admission procedure for doctoral studies in the PhD programme of Molecular and Cellular Biology, Genetics and Virology at the Faculty of Science, Charles University in Prague. The PhD application must be submitted by April 30, 2025.

To apply send your application including motivation letter, CV to [Please click the Apply button for the link or address].

If you have any further questions, please do not hesitate to contact Dr. Gabriela Pavlínková by e-mail gabriela.pavlinkova@ibt.cas.cz.

Publications to read:

• Bohuslavova R, Fabriciova V, Smolik O, Laura Lebron-Mora, Abaffy P, Benesova S, Zucha D, Valihrach L, Berkova Z, Saudek F, Pavlinkova G*. NEUROD1 reinforces endocrine cell fate acquisition in pancreatic development. Nature Communications 2023, 14 (1), 5554; https://doi.org/10.1038/s41467-023-41306-6
• ISL1 controls pancreatic alpha cell fate and beta cell maturation. R Bohuslavova, V Fabriciova, L Lebron-Mora, J Malfati, O Smolik, L Valihrach, S Benesova, D Zucha, Z Berkova, F Saudek, S Evans, Gabriela Pavlinkova*. Cell & Bioscience 2023, 13, 53. https://doi.org/10.1186/s13578-023-01003-9 .

More details on the processing of personal data are available on the website: https://www.ibt.cas.cz/cs/o-ustavu/uredni-deska/gdpr/

Tagged as: Life Sciences