We are offering a PhD position dedicated to the advancement of cryo-EM imaging methods at the interface of Structural Biology and Electron Imaging at the Forschungszentrum Jülich. Cryo-EM has become a very powerful method to visualize ice-embedded samples including purified proteins at close to atomic resolution. Single-particle cryo-EM as well as cryo-ET are based on conventional transmission electron microscopy (TEM) exposures taken in underfocus. In this project, we aim to further develop the imaging capabilities based on scanning transmission electron microscopy (STEM) including differential phase contrast as well as ptychography in order to apply them to vitrified biological specimens.
Your tasks include:
* Develop novel methods for the application of STEM methods to biological specimens.
* Operate high-resolution electron microscopes with ice-embedded biological samples.
* Perform image analysis to determine high-resolution cryo-EM structures of ice-embedded test specimens and in situ cellular samples.
* Employ advanced imaging methods together with an interdisciplinary team of physicists and structural biologists.
The Ernst Ruska Centre (ER-C) at the Forschungszentrum Jülich is one of the world-leading electron microscopy centres with more than 15 electron microscopes. The Structural Biology division of the ER-C investigates the structural and molecular mechanism of membrane biology and pushes the development of cryo-EM related methodology. Our main methods of investigation are single-particle electron cryo-microscopy (cryo-EM) as well electron cryo-tomography (cryo-ET) that we are also developing to advance existing imaging technologies towards high-resolution structural biology. The ER-C-3 (https://sachse.fz-juelich.de/), uses a comprehensive electron microscopy approach to study the biological structures of membrane-associated protein complexes. In close cooperation with experts in the development of methodologies in multidimensional STEM, new approaches for low-dose imaging at high contrast in Cryo-EM are explored both theoretically and experimentally.
Tagged as: Chemistry, Life Sciences
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