About Torbett Lab and projects
The laboratory of Dr. Bruce E. Torbett (www.torbettlab.org) is looking for a Senior Post Doctoral Fellow to work on projects related to the Structural and Biophysical Interactions of HIV Gag/Gag-Pol polyproteins with their host cell proteins during viral trafficking and assembly. This work aims to investigate how HIV co-opts cellular host machinery during active infection and identify the composition, dynamics, and function of novel host-viral interfaces that can be modulated for new therapeutic strategies. This work involves studies utilizing proximity labeling and quantitative proteomics, along with biochemical and virological validation of candidate cellular factors using CRISPR/Cas9 knockouts and confocal imaging techniques. Candidates must have a Ph.D. in Biochemistry or related field with documented experience (work or academic) in Mass Spectrometry-based Proteomics to identify host-factors of microbial infections, along with experience in target validation using relevant in vivo and in vitro assays.
The group works as a highly dynamic and interactive team and is part of the Center for Immunity and Immunotherapies (CIIT) of Seattle Children’s Research Institute (SCRI). Our studies are funded through a number of grants from the NIH (including the flagship HIVE Center). You will have the opportunity to interact and form collaborations with CIIT, SCRI and HIVE Center Investigators, as well as with collaborators throughout the Seattle area (University of Washington, Fred Hutch)
Seattle Children’s Research Institute and Torbett Lab offer competitive salaries, relocation assistance (if/as applicable), in addition to eligibility to a competitive benefits package as a full time employee
About the Position
– Develop scientific skills as appropriate for this training position as a postdoctoral fellow
– Meet with Principal Investigator on a regular basis to discuss proposed/ongoing studies, discuss implications of findings, and provide insights into new research directions
– Run research projects in an independent manner with oversight from Principal Investigator
– Write manuscripts, present data at national and international conferences
– Assist PI in writing progress reports, grant proposals, protocols as relevant to the postdoctoral fellow training
– Protocol development and review
– Excellent communication and writing skills
– Develop additional scientific skills as appropriate and carry out research/development activities of assigned research projects.
– Ability to learn and apply new technologies and skills
• Ph.D. in Cell/Molecular Biology related field
• Related and prior work or academic experience must include the following:
– Lentiviral vector production and gene delivery to primary cells
– Preclinical testing of lentiviral vector gene therapy in humanized mouse models
– Standard Virology assays (Neutralization, Titer, Packaging efficiency)
– Multicolor Flow Cytometry Analysis and Cell Sorting
– Cell culture of Primary Cells and Cell Lines (both adherent and suspension)
– Protein and DNA/RNA isolation and analysis
– Immunoassays, such as p24 ELISA
– Gene targeting with CRISPR/Cas9
– Ability to work in BSL-2+/BSL3 environments
– Fluorescence Microscopy
To apply, please visit our careers website, Requisition #2021-40236.
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Researchers work in close collaboration with one another, their colleagues at partner institutions including the University of Washington and Fred Hutch and our healthcare providers at Seattle Children’s Hospital, one of U.S. News & World Report’s top children’s hospitals. This collaboration is one of our key strengths, allowing our faculty to draw on a variety of disciplines and techniques as they pursue solutions to some of medicine’s most complex problems.
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Tagged as: Life Sciences
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