The laboratory of Prof. Dr. Daniela Duarte Campos at the Center for Molecular Biology (ZMBH) is seeking a PhD student in DNA damage, ER stress and cell division in bioprinted living tissues (f/m/d) For a collaborative project within the Cluster of Excellence 3D Materials Made to Order (3DMM2O) In bioprinting, there are two types of printing strategies: with nozzle (e.g. extrusion and inkjet) and nozzle-free (e.g. laser induced forward transfer (LIFT) and volumetric printing). During the printing process, cells are exposed to shear stresses, which are commonly observed in nozzle-based bioprinting. These can damage not only the cell membrane, but also impact cell structure and function at the longer term. Besides the impact of shear stress on cell function, the type of bioink and its polymerization (e.g. UV light) can trigger a change in cell phenotype that is harmful and potentially carcinogenic for cells. In this context, the impact of bioprinting technologies on DNA damage, endoplasmic reticulum (ER) stress, and cell division needs to be investigated at the molecular level. The Bioprinting Lab investigates biofabrication technologies and biomaterials (i.e. bioinks) suitable for tissue and organ engineering, and their impact on the structure and function of natural and synthetic living tissues. Your task will be to investigate the impact of nozzle-based and nozzle-free bioprinting methods on the DNA damage, ER stress and cell division in bioprinted 3D human living tissues. The work will involve a variety of techniques including but not limited to: • natural and synthetic bioink preparation and handling; • 2D and 3D cell culture of primary human cells and cell lines • bioprinting of human cells • histological and immunofluorescence staining, two-photon imaging, qPCR Your profile: • Master degree in Biology, Bioengineering, Biotechnology or related disciplines • Passionate about tissue and organ engineering • Previous experience in hydrogel handling, 3D (bio)printing, microscopy, cell culture, and/or microfluidic systems are preferred • Excellent achievements and English skills • Motivation to work in an international environment as part of a team We offer an international and attractive work environment within the Bioprinting Lab at the ZMBH and, as member of the 3DMM2O, you will have full access to modern state of-the-art infrastructure both at Heidelberg University and Karlsruhe Institute of Technology. The salary is based on TV-L scale. The position is limited to three years, with the possibility of extension. The position is available for immediate start. Applications should be sent to Prof. Daniela Duarte Campos (firstname.lastname@example.org) in one single PDF-file that includes a letter of motivation, CV, copy of transcripts and name of 2-3 referees. We ask for your understanding that application documents received by post will not be returned. Closing date for applications: June 30, 2021 Heidelberg University stands for equal opportunities and diversity. Qualified female candidates are especially invited to apply. Disabled persons will be given preference if they are equally qualified. Information on the application process and the collection of personal data is available at www.uni-heidelberg.de/stellenmarkt.
Tagged as: Life Sciences
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