qPCR Molecular Biologist, Scientist II Full-time

Job Title

Work Schedule: Standard (Mon-Fri)

Environmental Conditions: Cold Room/Freezers (-22degreesF/-6degrees C), Laboratory Setting, Office, Some degree of PPE (Personal Protective Equipment) required (safety glasses, gowning, gloves, lab coat, ear plugs etc.)

Job Description

Key Responsibilities:

• Design, develop, and optimize qPCR assays for gene expression, genotyping, copy number variation, and pathogen detection.
• Plan and execute experiments independently, ensuring data quality, reproducibility, and technical excellence.
• Perform nucleic acid extraction, cDNA synthesis, and quantification using standard molecular methods.
• Validate assay performance (sensitivity, specificity, and efficiency) and analyze results using platforms such as Applied Biosystems QuantStudio™ or Bio-Rad CFX Manager.
• Troubleshoot technical issues and implement improvements to enhance assay performance.
• Maintain detailed experimental records and prepare technical reports, SOPs, and validation documentation.
• Collaborate with product development, bioinformatics, and assay design teams to integrate molecular workflows.
• Ensure adherence to safety protocols, quality systems, and regulatory requirements (ISO, GLP, CLIA).

Qualifications:

Education: Bachelor's or Master's in Molecular Biology, Biochemistry, Genetics, or a related life sciences discipline. Experience requirements will vary based on degree.

Experience:

• Minimum of 1-4 years of hands-on experience performing qPCR. Experience requirements will vary based on degree.
• Prior experience with assay development, validation, and data analysis.
• Familiarity with nucleic acid extraction instruments is a plus.

Technical Skills:

• Understanding of molecular biology principles, qPCR kinetics, and assay troubleshooting.
• Strong analytical and problem-solving skills with a data-driven mindset.
• Strong understanding of qPCR principles, primer/probe design, and amplification kinetics.
• Proficiency in data analysis, statistical interpretation, and report generation.
• Experience working under quality and regulatory standards preferred.

Soft Skills:

• Excellent organizational, communication, and problem-solving skills.
• Ability to work independently as well as collaboratively in a multidisciplinary environment.
• Strong attention to detail and scientific curiosity.

Preferred Qualifications:

• Familiarity with bioinformatics tools for assay design.
• Experience with NGS.
• Experience with digital PCR (dPCR).
• Experience with qPCR multiplexing.
• Knowledge of automation and data management systems.