Scientist/Senior Scientist – Discovery RLT Full-time

Discovery Scientist

At Lilly, we unite caring with discovery to make life better for people around the world. We are a global healthcare leader headquartered in Indianapolis, Indiana. Our employees around the world work to discover and bring life-changing medicines to those who need them, improve the understanding and management of disease, and give back to our communities through philanthropy and volunteerism. We give our best effort to our work, and we put people first. We're looking for people who are determined to make life better for people around the world.

The Discovery Scientist will support the advancement of new ligands being developed for Eli Lilly's oncology pipeline. The role's primary duties are to provide technical scientific support for Lilly's early discovery programs, including but not limited to: characterization of new ligands in biochemical/biophysical assays, maintenance and expansion of mammalian cell culture banks, and protein conjugation and purification methods. This position will require accurate and detail-oriented execution of experimental work in close collaboration with on-site scientists and team members at other Lilly sites.

Roles and responsibilities

• Independently perform routine biochemical and/or cell-based assays to characterize the activity of early-stage ligands, including enzymatic activity assays, cytotoxicity assays, ELISAs, and functional reporter assays
• Passage and prepare mammalian cells to be used in assays and in vivo studies
• Determine binding affinity and kinetic parameters of protein-ligand interactions through biophysical techniques (SPR, BLI, DSF).
• Perform conjugation and purification of protein-based ligands by FPLC.
• Assist with sample processing (serum, tissue samples, etc) to support biodistribution and metabolite profiling
• Interface with external vendors to support early discovery activities.
• Critically assess data and methods, adapting and adjusting procedures as necessary.
• Fully adhere to Lilly Discovery Quality standards
• In conjunction with managers, establish priorities and meet timelines for key experiments.
• Provide clear communication of scientific data to co-workers through internal presentations and written methods.

Required Qualifications

• B.Sc. or M.Sc. in biochemistry, biology, chemistry, pharmacy, or related discipline
• 3+ years relevant postgraduate experience (industry preferred)
• Demonstrated success independently designing experiments and analyzing scientific data
• Experience performing in vitro biochemical/biophysical assays.
• Experience with mammalian cell culture and aseptic techniques.
• Strong background in performance of cell-based reporter assays and cytotoxicity assays.
• Familiarity with protein analysis and purification methods
• Ability to communicate scientific data effectively to an interdisciplinary team.
• Excellent problem-solving and decision-making skills.
• Working knowledge of MS Office, Graphpad Prism, and ChemDraw.

Additional preferences

• Experience with radiobinding assays or handling of radioactive materials is an asset.
• Experience with confocal microscopy, immunofluorescence and imaging of live/fixed cells is an asset.

Additional Information:

Physical Demands/Travel: The physical demands of this job are consistent with a lab environment.

Work Environment: This position's work environment is in a laboratory environment.