Sr. Scientist RNA Tx CGM Full-time

Sr. Scientist RNA Tx CGM

We are searching for the best talent for a Sr. Scientist RNA Tx CGM to be in San Diego, CA.

Position Summary

Scientific position within the Assay Development and Screening team developing assays to characterize oligonucleotides for target gene knockdown in relevant cell types, assess cell viability, screening for innate immune responses, and adapting assays to automation for high throughput screening of oligo libraries to identify hits and further characterize for potency and safety. Additionally, this position will provide analysis for PK/PD studies to test for siRNA levels and target mRNA levels in tissue samples.

You will be responsible for:

• Develop methods for RNA extraction, qPCR, immunoassays, and analytical methods to characterize oligonucleotides
• Conduct and troubleshoot experiments, independently and in collaboration with colleagues, to characterize and screen siRNA oligonucleotides through routine and consistent performance of 4 or more assays.
• Work with complex cell types including IPSC neurons for culturing and developing cell based functional assays
• Collect and evaluate data; prepare reports, summaries, and presentations; maintain and update laboratory notebook and the laboratory information management system
• Represent the ADS group on drug discovery project teams to plan for assay development and screening needs and updating the project team with resulting data, conclusions, and recommendations.
• Share work at team meetings and scientific forums for broader dissemination of results and contributions.
• Research literature and broaden scientific knowledge on relevant assay formats and disease areas, and strengthen expertise in oligo therapies.
• Support ADS team on operational lab functions, including managing inventories of supplies, ordering reagents and consumables
• Other activities may include serving as EH&S representative, DDI Champion, CREDO team member, or some other support role that helps the broader team.

Qualifications/Requirements

Required:

• A Bachelor's degree in Biology or related scientific field
• A minimum of 1 year in Complex cellular assays including primary cells and IPSC cells such as human neurons; Liquid handling automation; RNA extraction from cell cultures and tissues; QPCR, immunoassays; presentation skills; data analysis software such as Excel and Prism.

Preferred:

• Cell line generation; experience working with LIMS or databases; experience on cross functional matrix teams

Johnson & Johnson is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, disability, protected veteran status or other characteristics protected by federal, state or local law. We actively seek qualified candidates who are protected veterans and individuals with disabilities as defined under VEVRAA and Section 503 of the Rehabilitation Act.

The anticipated base pay range for this position is $105,000 to $169,050 annually.