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PRAFFUL SHARMA
What I've done
Highest degree
PhD
Current role
PhD Student
Skills
AlphaFold Circular Dichroism Cryo-EM EMSA (Electrophoretic Mobility Shift Assay) FPLC-SEC HPLC Isothermal Titration Calorimetry (ITC) manuscript editing and peer review Manuscript writing molecular cloning Molecular Dynamics Simulations mRNA in vitro transcription Polyribosome profiling Protein Crystallization Protein Purification Protein-Ligand Interactions Protein-Nucleic Acid Interactions Protein-Protein Interactions Pull down assay Site-Directed Mutagenesis TEM (transmission electron microscopy) Western Blot
Achievement(s)
Peer-reviewed publication(s) 1st author peer-reviewed publication(s) Conference Talks(s)
Contribution to Project (1)
Led optimisation and preparation of samples for cryo-EM datasets, and applied the cryoEM workflow independently to achieve high-resolution structures of the ribosome and ribosome complexes involved in translation from multiple model organisms.
Contribution to Project (2)
Designed and led multiple independent research projects involving biochemical as well as biophysical
assays.
Contribution to Project (3)
Carried out challenging MD simulations involving post-transcriptionally modified RNA and post-translational
modified proteins to compare the effect of modified RNA/protein on the overall structure and function.
What I'm looking for
Sector(s) of Interest
Academia
Role(s) of Interest
Postdoc
Research Fields & Keywords
Cell & Molecular Biology computational structural biology cryo-EM cryo-ET molecular biophysics RNA biochemistry Single molecule imaging
Supervision preferences
I like working independently and in a team, and I prefer weekly discussions about work updates or project-related ideas.
Career Goals
Group Leader/Faculty Start-up
Countries of Interest
France Germany Switzerland United Kingdom (UK) United States (US)
Remote requirements
On-site
Contact and Links
Email, LinkedIn, Google Scholar