Michael Krummhaar

PhD

Postdoc

and physiology • Molecular Biology: PCR Chromatography Cryo-EM Enzyme Assays fluorescence polarization heterogeneous protein expression and purification from bacterial insect cell culture Isothermal Titration Calorimetry (ITC) Mammalian and Bacterial Cell culture Mass photometry nano DSF Peptide Chemistry Protein Crystallization Surface Plasmon Resonance (SPR)

Conference Poster(s) Conference Talks(s)

Used genome mining to find novel bacterial glycosyltransferases and their target peptides. Generated the peptides in question by solid-phase peptide synthesis Determined the fold of these peptides using CD-spectroscopy Optimized bacterial expression of the glycosyltransferases as well as glycosyltransferase-peptide combinations for maximum yield and activity Obtained the crystal structure of 4 glycosyltransferases. One with the target peptide within the active site.

Identified the efficiency of different E2 ubiquitin ligases to ubiquitylate a neo-substrate Performed all steps of CryoEM sample preparation in order to obtain the fully assembled ubiquitylation-complex Solved 2 CryoEM structures of CRL-ligases in complex with a PROTAC and neosubstrate. Solved a crystal structure of a neosubstrate-PROTAC-Substrate acceptor complex

Optimized insect cell expression for a challenging protein. Generated new constructs when necessary. Helped guide ligand design by ITC and FP. Established a TR-FRET assay for high-throughput screening of ligands for induced-proximity therapeutics. Prepared samples for CryoEM System administrator for the local HPC cluster.