Brent Vasquez

PhD

Postdoc

BCA Protein Assay Immunoassays immunocytochemistry In Situ Hybridization Independent project management Machine Learning (ML) Project Management Protein Purification RNAseq scRNA-Seq (Single-cell RNA Sequencing) SEM (scanning electron microscopy) TEM (transmission electron microscopy) Western Blot

1st author preprint(s)

• University-wide Pitt Three Minute Thesis (3MT) competition winner for presentation – ‘Directing enamel development: How phosphorylated amelogenin (AMELX) Creates Strong Enamel’ (2025) • Ruth L. Kirschstein Predoctoral Individual National Research Service Award (F31 NRSA) from NIH for dissertation proposal – ‘The impact of amelogenin phosphorylation on biological processes within the tooth organ’ (2024) • K. Leroy Irvis Fellowship from the University of Pittsburgh for record of distinguished academic achievement (2024) • AADOCR Student Research Day Travel Award for research poster presentation (2024) • Enamel 10 New Investigator Award for research poster presentation (2022) • The Valley Hospital Auxiliary Scholarship for high grade point average and academic achievement (2014)

• Amelogenin phosphorylation affects key regulatory genes in the enamel organ Vasquez B, Bui AT, Priya Mullick, Lasya Bhogadi, Margolis H, Beniash E. Calcified Tissue International. 2026. • Amelogenin phosphorylation affects protein-protein interactions in vivo Vasquez B, Bui AT, Margolis HC, Beniash E. Calcified Tissue International. 2026. • Ethical, Robust and Accurate use of Artificial Intelligence (AI) in Animal Research Vasquez B, DeRicco J, Yates B, Cunningham R. Nature Lab Animal. 2025. • Role of amelogenin phosphorylation in regulating dental enamel formation Gabe C, Bui AT, Lukashova L, Verdelis K, Vasquez B, Beniash E, Margolis HC. Matrix Biol. 2024. • Identification of stages of amelogenesis in the continuously growing mandibular incisor of C57BL/6J male mice throughout life using molar teeth as landmarks Bui AT, Lukashova L, Verdelis K, Vasquez B, Bhogadi L, Gabe CM, Margolis HC, Beniash E. Front Physiol. 2023. • Elucidating the role of keratin 75 in enamel using Krt75tm1Der knock-in mouse model Deshmukh R, Vasquez B, Bhogadi L, Gabe CM, Lukashova L, Verdelis K, Morasso MI, Beniash E. Front Physiol. 2022. • The phosphorylation of serine55 in enamelin is essential for murine amelogenesis Dong C, Lamichhane B, Yamazaki H, Vasquez B, Wang J, Zhang Y, Feng JQ, Margolis HC, Beniash E, Wang X. Matrix Biol. 2022.

• AI and Machine Learning Internship | University of Pittsburgh Office of Research Protections (2024 – 2025) • Mentor/Internship | University of Pittsburgh Graduate Global Ties (2023) • Student Representative | School of Dental Medicine Dean's Advisory Board (2023 – 2025) • Organizing Committee Member | Pittsburgh Craniofacial Sciences Training Program (2023 – 2025) • Internship | Valley Hospital Career Frontiers Program (2009)