Research Associate – Steif Lab Full-time

Research Associate – Steif Lab

The Steif Lab and collaborating research groups are located in the Department of Basic and Translational Research at the BC Cancer Research Institute in Vancouver. The successful candidate will report directly to the Principal Investigators. The incumbent may also assist with technical training, supervision and mentorship of trainees and staff.

Job Summary

We are developing and applying new single cell omics methods for tracking tumour clonal trajectories, characterizing cellular states, and assessing interactions with the microenvironment in patient tissues. Our researchers use the latest single cell technologies to rapidly identify genes and pathways involved in cancer development and progression. This position will contribute to multiple research projects currently ongoing, working within the Single Cell Genomics Platform which is located across two separate research facilities in Vancouver, BC, Canada. The successful candidate will provide technical expertise, contribute to protocol optimization and technology development, and carry out high-throughput data generation.

We are seeking a highly motivated and highly skilled individual with expertise or experience in single-cell genomic library preparation, liquid handling robotics, and/or next-generation sequencing, with a strong background in molecular biology. The successful applicant will work under the direction of Dr. Adi Steif, collaborating Principal Investigators including Drs. Pamela Hoodless and Samuel Aparicio and their designates. This position will provide the candidate with opportunities to contribute to high-impact single-cell multiomics and translational cancer research within a multidisciplinary and collaborative environment. The position start date is flexible and certain part-time arrangements (e.g. working full-time 4 days per week) may be considered.

Work Performed

• Plan and perform experiments utilizing established complex procedures and/or techniques, including the use of in-house liquid handling robotic platforms and commercial single cell platforms.
• Work together with the PIs and team members to support the development of new wet-lab protocols and design rigorous technical experiments.
• Optimize, troubleshoot and carry out sensitive experiments, including processing of primary patient biospecimens.
• Develop and implement standard operating protocols (SOPs).
• Process diverse samples, including primary tissues, for dissociated and spatial single cell omics profiling.
• Prepare libraries and run next generation sequencing platforms.
• Visualize and analyze samples using microscopy and flow cytometry.
• Maintain detailed laboratory records, including using a LIMS system to track and record sample and experimental data.
• Provide technical expertise and support in tissue culture, molecular biology, and biochemistry.
• Assist in general lab maintenance such as reagent preparation, ordering, receiving and tracking inventory for lab supplies.
• Contribute to analysis and interpretation of data resulting from high-throughput experiments.
• Stay up-to-date on the latest scientific developments and new commercial platforms/protocols, informing the PIs of new opportunities and directions.
• Provide regular updates to Dr. Steif and collaborating PIs to problem-solve and plan research directions.
• Present regular updates at lab/team meetings and scientific meetings.
• Write reports and contribute to writing ethics applications, academic manuscripts, and grant proposals.
• Carry out any other related duties as assigned or required in keeping with the qualifications and requirements of this academic level.
• Provide technical training, supervision and mentorship to trainees and staff.

Consequence of Error/Judgement

The work is complex and requires a high level of training, tasks are assigned and the incumbent exercises a considerable amount of responsibility, initiative and judgment in determining their work schedule to achieve those tasks. Errors may result in the permanent loss of irreplaceable tissue samples, loss of thousands of dollars of material and/or damage to equipment. Incorrect decisions or actions may damage the reputation of the laboratory, lead to a loss of credibility in this field, and be financially costly. Noncompliance with biosafety and chemical safety regulations may also result in the closure of the facility or loss of grant funding.

Supervision Received

The successful applicant will work under minimal supervision. From time to time, Principal Investigators or their Laboratory Research and Operations Manager(s) may provide specific instructions on unusual problems or new project directions. This position will report to the Principal Investigators.

Supervision Given

Depending on experience and expertise, the successful applicant may be required to assist in the training and supervision of graduate students/trainees, other staff, or external collaborators; including providing instruction on the use of lab equipment and procedures, and will help others troubleshoot experiments in their area of expertise.

Qualifications

• A PhD in the molecular biology, genetics or related fields, or a PhD and postdoctoral academic or industry work experience in the aforementioned areas.
• Past experience with single-cell genomics, liquid handling robotics, cell isolation and/or next-generation sequencing library preparation is strongly preferred.
• Working hands-on experience in tissue handling and tissue dissociation methods is an asset.
• Ability to perform and troubleshoot a wide variety of molecular and cellular biology techniques, such as cell culture, microscopy, RNA/DNA isolation, quantitative PCR, flow cytometry, immunohistochemistry, protein isolation, and western blotting.
• Demonstrated understanding of experimental design and assay optimization.
• A record of publication in peer-reviewed journals.
• Past experience with basic bioinformatic analysis in Python or R is considered an asset.
• Ability to work independently and part of a collaborative, multi-disciplinary team.
• Willingness to respect diverse perspectives, including perspectives in conflict with one's own.
• Demonstrated commitment to enhancing one's own awareness, knowledge, and skills related to equity, diversity, and inclusion.
• Leadership qualities, strong interpersonal skills, communication skills (oral and written) and mentorship/teaching ability.
• Excellent organizational skills, conscientiousness and attention to detail.
• Ability to multi-task, prioritize and handle diverse project assignments concurrently.

An application package should include:

• Cover letter outlining the applicant's past research experience and strengths as they relate to this position;
• A detailed curriculum vitae;
• The names and contact information of three (3) referees