Research Technician – College of Medicine and Health – 107950 – Grade 4 Part-time

Job Title

College of Medicine and Health – Infrastructure

Job Description

Location: University of Birmingham, Edgbaston, Birmingham UK

Full time starting salary is normally in the range £27,546 to £29,171 (pro-rata for part time)

Grade: 4

Full Time, 0.5 FTE (17.5 hours per week)

Fixed Term contract up to September 2029

Background

This part-time position (17.5 hours/week) until 30th September 2029 within the College of Medicine and Health, School of Medical Sciences, Department of Cardiovascular Sciences and will be supporting the research of Julie Rayes and Alex Slater.

This post is funded by the Rosetrees Trust with the aims of developing new therapies combatting sepsis. Here we will produce and characterise inhibitors of calprotectin. We will use its three major receptors, CD36, RAGE and TLR4, as templates to develop truncated proteins and peptide inhibitors. These will be characterised for their ability to bind calprotectin and inhibit the procoagulant and inflammatory responses.

Role Summary

You will provide technical support for research activities as required, utilising your technical skills and a wide range of techniques. You will also be expected to ensure your working area is safe and support others in following health and safety instructions. You may need to operate out of normal working hours to accommodate research activity. In order to flexibly use resource you may be given the opportunity to work in different areas of the department or wider University.

Main Duties

The role holder will provide support for the research undertaken by Professor Rayes and Dr Slater using a variety of techniques to include, but not exclusively:

• Protein production
• Protein expression using mammalian and bacterial culture systems
• Protein purification using affinity chromatography and size exclusion chromatography
• Protein-protein interaction assays including surface binding assays (ELISA)
• Molecular biology techniques focussing on:
• Site directed mutagenesis
• Mini prep and maxi prep DNA extraction
• Cell culture, including working with blood cells and blood cell assays
• Flow cytometry
• Carry out techniques and experimental procedures as required by research staff, students and Principal Investigators, designed to contribute to a specific research project or projects.
• You will be expected to resolve problems in your area of expertise but also to recognise when problems need to be referred to more senior staff.
• You will need to maintain accurate records of all techniques and experimental procedures carried out, and of any other research activities you may be involved in, including results.
• Comply with any legislation relevant to the area of research/activity.
• Comply with the Standard Operating Procedures for the activity.
• Demonstrate techniques to other staff and/or students on the area of expertise as required.
• You will contribute to internal and external audit procedures and to regulatory inspections.

Health and Safety

• You will be expected to uphold compliance with all University and local Health & Safety policies, including risk assessments and manual handling, reporting any breaches or non-compliance to the relevant manager.
• You will be expected to manage the safe handling and disposal of all materials used in research laboratories, including hazardous materials.
• If required, assist or prepare risk assessments and keep these up to date.
• You will need to maintain all records as appropriate.
• Carry out regular maintenance processes as appropriate.

Administrative Duties

• You may be required to assist with PAT testing and calibration of equipment, and with updating equipment inventories.
• Maintain stocks of consumables and, if necessary, creating purchase order requisitions.
• Receipt and correct storage of consumables and chemical reagents.
• Ensure that the facilities are maintained in a clean, functional and safe condition, reporting any problems.
• Reporting any problems with equipment or services which cannot be resolved to the line manager as appropriate.
• Adopt a proactive attitude to assisting with and implementing carbon reduction and energy saving measures where practicable.
• Supports equality and values diversity, moderates own behaviour to avoid unfair discriminatory impact or bias on others.
• Any other duties commensurate with the grade and skill base of the post holder.

Required Knowledge, Skills, Qualifications, Experience

• NVQ, BTEC/ONC at level three or equivalent level three qualification and specific relevant experience in an appropriate subject.
• GCSE level 2 (A*- C/4-9) or equivalent level 2 qualification in mathematics and English.
• Practical experience of the above techniques and a working knowledge of Good Laboratory Practice.
• Experience of working in a laboratory based teaching or research environment; or be able to demonstrate the capability to participate in research projects.
• Understanding of Human Tissue Act
• Ability to organise and manage own workload after consultation with manager.
• Familiarity with the use of a wide range of general laboratory equipment e.g. balances, pH meters, autoclaves, glass washing machines, microscopes.
• Proactive approach to Health and Safety matters, together with the communication skills to interact with a range of people, including students.
• Ability to be flexible to meet deadlines.
• The post-holder will be fully familiar with the safe handling and storage of laboratory materials.
• Good IT skills, having a familiarity with both Windows and/or Apple Macintosh OS will be advantageous.
• Understands the importance of equality and diversity in the workplace.
• Ability to identify and respond to equality and diversity issues in line with relevant policies and procedures.