A Postdoctoral position is available in the Vecchio Lab at the University of Nebraska—Lincoln (UNL) for an NIH-funded project that aims to clarify the structural and functional biology of human membrane proteins and membrane protein complexes involved in epithelial cell/cell adhesion and intercellular transport. We use X-ray crystallography, cryogenic electron microscopy (cryo-EM), microcrystal electron diffraction (microED), and small-angle X-ray scattering (SAXS) to elucidate structures of these molecules. We also employ protein engineering and Fabs to enable these structural biology methods. The candidate will be responsible for construct design, protein expression and purification, biophysical protein/protein interaction measurements, and structure determination of these target proteins. Other position activities include assisting with lab organization and operation, student and other personnel training, and grant proposal development and writing, including postdoc fellowships.
The candidate will learn comprehensive methods associated with the preparation of human membrane proteins and complexes for structural studies, biophysical techniques to measure protein/protein interactions, and membrane protein-specific skillsets to complement their existing crystallography expertise. Through a two-year NSF-funded training fellowship, potential exists for the candidate to learn cryo-EM first-hand at one of the NIH national cryo-EM service centers, in order to use this technique to determine target protein structures.
Will have a Ph.D. in structural biology, molecular biology, biochemistry, biophysics, or a related discipline. The ideal candidate will have some experience in membrane protein preparation and handling and proficiency in one structural biology technique, preferably X-ray crystallography. Such a candidate will be enabled to make immediate and impactful contributions to the lab and field, as we have an established and well-characterized system up and running. The candidate should also possess effective written, verbal, and listening skills.
The Vecchio Lab is housed in the Department of Biochemistry on the UNL City Campus. We are a multidisciplinary group with expertise in membrane protein crystallography (including LCP), and membrane protein biochemistry and biophysics. For crystallography, we have an Art Robbins Gryphon robot for VD and LCP crystallization, a CrysCam UV imager with Cy3 filter, and four incubators ranging from 4-20°C. For BLI we have Sartorius/FortéBio Octet R8 and BLItz interferometers, and for MST we have a NanoTemper Monolith. Other technologies include: FSEC, CD, DSC, and ITC, and we have capabilities in detergents, nanodiscs, saposin, amphipols, and SMALPs.
Structural Biology Infrastructure
About UNL, Lincoln, and Nebraska
UNL is a Carnegie designated R1 Research University (very high), the flagship of the NU system with 26,000+ students, and a member of the Big10 Academic Alliance. Nebraska ranks #9 in The Best States Ranking (US News and World Report) and #17 in America’s Best States to Live In (MSN). Lincoln is Nebraska’s capital, the 2nd most populated NE city and the 70th most populated in the US (320,000 people). Lincoln offers a low cost of living; low unemployment, crime, and poverty rates; great public schools, housing affordability, and bike infrastructure; high quality of life; and wide-open spaces. It’s a fantastic place to raise a family, own a home, go for a bike ride, and maintain a work-life balance. And oh, the sunsets!
Interested applicants can apply by emailing Dr. Vecchio using “Postdoc: Vecchio Lab” in the Subject. Please send a CV containing contact info for 3 references and a cover letter addressing: 1) reason for applying, 2) past and current research experience, 3) future research/science/career ambitions (non-academic welcome), and 4) training and mentoring expectations.
Approximately March 1, 2021, with secure funding until 2025 via an NIH grant from NIGMS. Applications will be reviewed as received until the position is filled.
As an EO/AA employer, qualified applicants are considered for employment without regard to race, color, ethnicity, national origin, sex, pregnancy, sexual orientation, gender identity, religion, disability, age, genetic information, veteran status, marital status, and/or political affiliation. See https://www.unl.edu/equity/notice-nondiscrimination.
Tagged as: Life Sciences
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