Taiwo OLAJIDE

PhD

PhD Student

2D and 3D cell culture systems Animal Handling bash scripting bioinformatics workflows BioRender BSL-2 lab experience Bulk RNA-seq Chromatin Immunoprecipitation (ChIP) Confocal Microscopy CUT&RUN DNA/RNA extraction ELISA FACS (Fluorescence-Activated Cell Sorting) Flow Cytometry Fluorescence Microscopy Graphpad Prism HaploView Hi-C Sequencing High Performance Computing (HPC) Image J immunocytochemistry Multi-Omic Integration PLINK SPSS TapeStation Bioanalyzer Transwell in vitro Models

Peer-reviewed publication(s) 1st author peer-reviewed publication(s) Conference Poster(s)

Designed and executed a mechanobiology-driven research project to decode how constricted migration through the tumor microenvironment (TME) induces epigenetic memory and 3D genome reorganization in metastatic melanoma, directly informing therapeutic targeting strategies. • Epigenomics & Target Discovery: Designed and optimized CUT&RUN protocols to profile histone modifications and characterize epigenetic reprogramming events associated with constricted migration in A375 melanoma cells, identifying candidate chromatin targets with therapeutic relevance. • 3D Genome Architecture: Executed Hi-C experiments and downstream bioinformatics analysis to map genome compartment shifts (A/B compartments) induced by mechanical constriction, elucidating how the physical TME drives stable transcriptional reprogramming and metastatic phenotype. • Multi-Omics Data Integration: Integrated multi-omics datasets (Hi-C + CUT&RUN + RNA-seq) to correlate chromatin state changes with transcriptional output, generating mechanistic insights into H3K27me3-mediated migratory efficiency in high-metastatic melanoma. • Scientific Communication: Presented research findings at Cold Spring Harbor Laboratories Genome Organization Meeting (2024) and FASEB Mechanobiology of the Nucleus Conference (2025), demonstrating ability to communicate complex science to expert audiences.

Led a translational public health genomics research program investigating the genetic basis of diseases prevalent in sub-Saharan Africa, including CKD, hypertension, malaria, and keloids, applying population genetics tools to clinically actionable questions. • Genomic Epidemiology: Designed genotyping protocols to investigate SNP-disease associations (CKD, hypertension, malaria, keloids) in Nigerian populations using PLINK-based GWAS pipelines, generating publishable population-level insights.

Designed and executed protocols to investigate TNFA gene SNPs associated with malaria severity in pediatric populations, combining molecular genotyping with large-scale epidemiological data analysis. • Data Management: Managed, curated, and analyzed large experimental datasets; prepared findings for presentation to multidisciplinary research teams and for peer-reviewed publication.