Anugreha Mohan

Master

MSc Student

and physiology • Molecular Biology: PCR and publishing research on microbial genetics and host-microbe interactions. • Molecular & Microbial Techniques: Proficient in DNA/RNA extraction Bioinformatics Pipelines cell and molecular biology techniques Cell Culture DNA and RNA isolation and quantification Proefficieny in R and Python RNA Sequencing and Data Analysis

For my MSc thesis at IISER Thiruvananthapuram, I developed a computational pipeline to predict cell-type-specific synthetic lethal gene pairs in human cancers using single-cell RNA sequencing data. I analyzed gene expression patterns, integrated gene interaction network information, and used CRISPR knockout datasets to filter and prioritize candidate gene pairs for their potential therapeutic relevance. I also optimized pipeline execution using parallel workflows.

During a six-month research project at the Indian Institute of Science (IISc), Bengaluru, I investigated epithelial-to-mesenchymal transition (EMT) in liver cell populations during development and disease. I processed and analyzed public single-cell RNA sequencing and bulk transcriptomic datasets using Python and R, examined EMT-associated gene expression signatures, and compared cellular populations to investigate changes in cell states.

During my research internship at the Jhaveri Microbiology Centre, LV Prasad Eye Institute (LVPEI), I worked with clinical isolates to investigate antimicrobial resistance patterns. My work involved DNA and RNA isolation, PCR and qPCR, minimum inhibitory concentration (MIC) assays, and mammalian cell culture. This experience provided hands-on training in molecular biology techniques and microbiological research.