Research Technician Position Full-time

Job Summary:

The Kostova laboratory at Carnegie’s Department of Embryology in Baltimore is searching for a full-time research technician. This is a great opportunity to work with a postdoctoral fellow using cutting edge technologies to answer fundamental questions of cell biology. Ideal candidates should have a B.S. in Biology or related field, experience in modern molecular biology techniques, a strong work ethic, and be able to join the position for at least 2 years. 

We are investigating the fundamental question of how cells respond when their ribosomes break down. The ribosome is one the most complex and long-lived molecular machines in the cell and the presence of functional ribosomes is essential to live. Ribosome damage has been recently linked to complex human diseases, such as cancer and neurodegeneration. We use cutting-edge technology, such as CRISPR-mediated genome editing and genome-wide screens, to model and map the molecular pathways that ensure ribosome integrity.

We are looking for a highly motivated individual with strong organizational skills to join our team and help explore these novel areas of biology.

Key Responsibilities:

• Grow and clone mammalian cell lines (tissue culture)
• Perform CRISPR-mediated genome editing and characterize the obtained mammalian cell lines (genotyping, qPCR, Western blotting, FACS analysis)
• Isolation of ribosomes and RNA analysis (sucrose gradient fractionation, immunoprecipitation, RNA purification and electrophoresis)
• Perform high-throughput genetic screens and prepare libraries for Illumina sequencing

Requirements:

• B.S. in Biology (or equivalent)
• Lab experience
• Strong interpersonal skills and ability to work as part of a team

Preferred Skills:

• Mammalian tissue culture
• Modern molecular biology techniques, e.g., DNA/RNA purification, cDNA synthesis, qPCR, plasmid cloning, SDS­PAGE, and Western blotting
• Great organizational skills
• Excellent written and oral communication skills

If interested please send the following documents to kostova@carnegiescience.edu:

• Cover letter
• Curriculum Vitae
• Academic transcript
• Recommendation letters (at least 2)

Tagged as: Life Sciences