Postdoctoral Research Assistant in single-molecule biophysics of eukaryotic transcription Full-time

Postdoctoral Research Assistant in Single-Molecule Biophysics of Eukaryotic Transcription

Applications are invited for a Postdoctoral Research Assistant in Experimental Biophysics to join the Nynke Dekker Lab in the Department of Physics at the University of Oxford. The post is available initially for a fixed-term duration of 3 years. We are looking to hire an outstanding experimentalist for an exciting project at the interface of biophysics and biochemistry that has as its goals to understand the response of RNA polymerase to DNA damage-induced transcription stress. Unperturbed gene expression is vital for correct functioning of cells. Eukaryotic RNA polymerase II is the enzyme that transcribes genes into mRNA, enabling subsequent protein synthesis. Its transcriptional activity is continuously challenged by the occurrence of DNA damage, resulting from exposure of DNA to cellular metabolites and environmental agents. Bulky and helix-distorting DNA damage physically blocks the forward movement of transcribing RNA polymerase II. Such DNA damage-induced transcription stress can lead to severe cellular dysfunction, death and senescence, and is believed to be a major contributor to aging-related diseases. You will perform biophysical experiments to unravel the molecular mechanisms behind these aging-related phenotypes. A willingness to learn and employ biochemical approaches and interact with external collaborators in molecular biology and biochemistry at the University of Oxford and in the Netherlands is essential. In doing so, you will publish high-quality scientific papers to advance this exciting field.

The Nynke Dekker Lab is a highly successful single-molecule biophysics research lab based at the University of Oxford. The lab focuses its studies on understanding DNA replication and related molecular motor-based processes from a quantitative perspective in vitro. The lab employs state-of-the-art biophysical techniques (e.g. advanced single-molecule fluorescence microscopy, optical and magnetic tweezers) together with in-house molecular biology and biochemistry. Performing experiments at the single-molecule level requires broad and integrated expertise; as such, we employ outstanding international scientists trained in biophysics, biochemistry and cell biology who work together enthusiastically as a multidisciplinary team. You will be part of this group and will interact actively with current members.

Applicants should hold a PhD or be close to completion in quantitative biochemistry or biophysics. The post holder should have a strong background in quantitative experimental science. Previous experience in single-molecule biophysics, fluorescence microscopy, protein biochemistry, or related areas will be an advantage. The successful candidate is expected to demonstrate strong experimental and quantitative skills, scientific independence, excellent organizational abilities, and effective interpersonal communication skills. They should have an interest in interdisciplinary research and a willingness to work at the interface of biophysics, biochemistry, and structural biology. The ability to work both independently and collaboratively within a multidisciplinary team is essential.